Seek? In the grand scheme of things asking forgiveness only applies if you're going to not be that transformative and something like YouTube's automated copyright strikes might affect you. "Ask Forgiveness" is often a better option.
Fair use is a defense, not a requirement - You don't need permission to claim fair use; it's a legal defense if you're sued
Seeking permission can backfire - Copyright holders may deny permission even when fair use would apply, creating unnecessary barriers.
This is especially true for parody and commentary.
The difference between Seek and Ask Forgiveness in the situation outlined is that Seek lays out costs before hand and generally they are minimal, and Ask Forgiveness can determine costs at the will of the person sampled or remove the work completely from circulation.
Fair use is a defense, not a requirement - You don't need permission to claim fair use; it's a legal defense if you're sued Seeking permission can backfire - Copyright holders may deny permission even when fair use would apply, creating unnecessary barriers.
This is especially true for parody and commentary.